Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24804^* Cite

Description

Differential response of lamellipodial actin network to latrunculin a (LA), a binder of monomeric actin. This electron micrograph of a Xenopus keratocyte)lamellipodium treated with LA (0.1 μM for 30 min) reveals actin depletion from the lamellipodial rear. A similar image of an LA treated fibroblast is available as CIL 24805. Image corresponds to Figure 7d from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: keratocyte
Cellular Component: actin cytoskeleton; lamellipodium

Biological Context

Biological Process: branching of actin filaments; latrunculin a treatment

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL24804
Archival Resource Key (ARK): ark:/b7295/w9cil24804

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Visualization Methods: shadowing and plating; platinum replica
Processing History: unprocessed raw data

Sample Preparation

Methods: permeabilized tissue; glutaraldehyde fixed tissue; phalloidin
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	769px	0.017µm
Y	1226px	0.017µm