Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24784^* Cite

Description

Localization of XAC (Xenopus ADF/cofilin) in Xenopus fibroblasts. Immuno-EM with XAC antibody at low magnification. High magnification view is available at CIL 24785. Nucleus and surrounding regions look bright because of high content of cellular proteins, which creates high electron density (brightness in inverted contrast). Immunogold-labeling in these central regions is very low. Image corresponds to Figure 10h from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: fibroblast
Cellular Component: lamellipodium; Xenopus ADF/cofilin; nucleus

Biological Context

Biological Process: actin filament organization; ruffle organization
Molecular Function: structural constituent of cytoskeleton

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL24784
Archival Resource Key (ARK): ark:/b7295/w9cil24784

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating; platinum replica; primary antibody plus labeled secondary antibody; immuno gold
Processing History: unprocessed raw data

Sample Preparation

Methods: permeabilized tissue; glutaraldehyde fixed tissue; polyethylene glycol
Relation To Intact Cell: dispersed cell in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2753px	——
Y	1878px	——