Alternate header for print version


Licensing
This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:22706*  Cite 
Description

Lattice diffusion of CLIP-170(H2)–GFP protein (a carboxyl truncation of the plus end microtubule tracking protein CLIP-170). The movie shows pseudocolored overlaid images of rhodamine-labeled microtubules (red) and GFP-tagged CLIP-170(H2) protein (green). In the absence of EB1, CLIP-170(H2) binds along the length of the microtubule lattice and exhibits diffusive movement (blue arrowhead). (Scale bar, 2 micron.) Microtubule dynamics were visualized at 22 °C using a TIRF microscope fitted on an inverted microscope (Nikon TE-2000U). The laser light intensities used for imaging were kept low, so that the photobleaching rates were slow compared with the dwell times. Images were captured with a backilluminated electron-multiplying CCD camera (Cascade-512B, Photometrics) using time-lapse capture at burst mode at 10 frames per second for the analysis of +TIP dynamics at the single-molecule resolution. Movie corresponds to video S2 from Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):492-7. Epub 2009 Jan 6.

Biological Sources
Cellular Component
microtubule
microtubule plus end
CLIP-170(H2)
Biological Context
Biological Process
microtubule polymerization or depolymerization
Attribution
Names
Ram Dixit
Brian Barnet
Jacob E. Lazaru
Mariko Tokit
Yale E. Goldman
Erika L. F. Holzbaur
Published
Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):492-7. Epub 2009 Jan 6.
Pubmed
18227130
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL22706
Archival Resource Key (ARK)
ark:/b7295/w9cil22706
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
TIRF
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Rhodamine
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
in vitro reconstitution
Relation To Intact Cell
cell free
Dimensions
Spatial Axis Image Size Pixel Size
X 345px 0.033µm
Y 145px 0.033µm
Time 0.1 seconds 194