Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:2^* Cite

Description

The distribution of the mitochondria (labeled with antibody to hsp70) in relation to intermediate filaments (labeled with vimentin antibody) 8 hours after release from serum starvation in methanol-fixed 3T3 fibroblasts. Microscope - Zeiss Axioplan I Illumination - Mercury arc lamp Objective - 100X oil/1.3 NA Intermediate lens between obj and camera - 0.63X Camera - Axiocam MRM CCD Filters: 1. Ex 485/20, Em 515-565 BP, dichroic 510 2. Ex 560/40, Em 630/60, dichroic 595

Technical Details

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Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: fibroblast
Cellular Component: type III intermediate filament; mitochondrion

Biological Context

Biological Process: intermediate filament-based process; mitochondrion morphogenesis
Molecular Function: cytoplasm organization; ATP biosynthetic process

Attribution

Names: Linda Parysek; Trudy Aebig

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL2
Archival Resource Key (ARK): ark:/b7295/w9cil2

Imaging

Image Type: microscopy
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: localized accumulation of fluorescent probe
Visualization Methods: Alexa Fluor 594; Alexa Fluor 488; primary antibody plus labeled secondary antibody
Processing History: unprocessed raw data

Sample Preparation

Methods: methanol fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1300px	104nm
Y	1030px	104µm