Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:187^* Cite

Description

The image shows a developing Drosophila spermatid immunostained for caspase activity (green). TRITC-phalloidin is used to label actin (red). Caspase is required in spermiogenesis for the elimination of unwanted organelles and cytoplasm via apoptosis.

Technical Details

See E. Arama et al. 2003 Caspase activity and a specific cytochrome C are required for sperm differentiation in Drosophila. Dev. Cell 4:687-697.

Biological Sources

NCBI Organism Classification: Drosophila melanogaster
Cell Type: spermatid
Cellular Component: cytoplasm

Biological Context

Biological Process: spermatogenesis

Attribution

Names: Hermann Steller

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL187
Archival Resource Key (ARK): ark:/b7295/w9cil187

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: Tetramethyl rhodamine (TRITC)
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue; ethanol fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3200px	——
Y	4000px	——