Fluorescence images of control and Si-RNA transfected HeLa cells treated with nocodazole and allowed to recover. Mitotic cells were stained for alpha-tubulin (top row, green) or NuMA (center row, red). The merged images include DAPI staining of DNA (blue). Cohesin depletion inhibits spindle recovery. Cells were observed with an Olympus IX70 microscope and recorded with a Magnaview CCD camera. See Fig 4B in Kong et al., 2009, Mol Biol Cell 20:1289-1301.