Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:1585^* Cite

Description

Cultured chicken embryonic cardiomyocyte transfected with GFP control vector in the nucleus, stained with alpha-actinin at the Z-lines in red and Tropomodulin1 (at the pointed ends of the actin filaments) in blue. Epifluorescence imaged on a Zeiss Axioplan microscope.

Biological Sources

NCBI Organism Classification: Gallus gallus gallus
Cell Type: early embryonic cell; cardiac muscle cell
Cellular Component: striated muscle thin filament; actin cytoskeleton; F-actin capping protein complex; Z-line components; tropomodulin; alpha-actinin

Biological Context

Biological Process: striated muscle contraction; striated muscle organization; striated muscle development

Attribution

Names: Caroline McKeown

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL1585
Archival Resource Key (ARK): ark:/b7295/w9cil1585

Imaging

Image Type: widefield illumination
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission; distribution of epitope
Source of Contrast: compartmentalization of stain or label; distribution of epitope; distribution of a specific protein; distribution of a specific nucleic acid sequence
Visualization Methods: EGFP; Alexa Fluor 546; Alexa Fluor 405

Sample Preparation

Methods: formaldehyde fixed tissue; detergent permeabilized

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1024px	0.067µm
Y	1024px	0.067µm