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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:15795*  Cite 
Description

Single-plane sequences of anucleate, centrosome-containing cytoplasts (sand dollars) expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). This anucleate cytoplast has two centrosomes, farther apart than the distance to the equator. A furrow initiates at the expected time and place, and proceeds to completion. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. Video corresponds to Fig 7b and the second segment of video 6 in J Cell Biol. 2009 Dec 14;187(6):831-45.

Biological Sources
NCBI Organism Classification
Dendraster excentricus
Cell Line
anucleate cytoplast
Cellular Component
microtubule
ensconsin
centrosome
Biological Context
Biological Process
cytokinesis after mitosis
Attribution
Names
George von Dassow
Koen J.C. Verbrugghe
Ann L. Miller
Jenny R. Sider
William M. Bement
Published
J Cell Biol. 2009 Dec 14;187(6):831-45.
Pubmed
20008563
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL15795
Archival Resource Key (ARK)
ark:/b7295/w9cil15795
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.32µm
Y 512px 0.32µm
Time 10 seconds 180