Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:147^* Cite

Description

Phase contrast image of a PTK-1 cell expressing constitutively active Rac1(Q61L). Live cells were visualized using X-rhodamine tubulin (injected at 1 mg/mL)and eGFP-Rac1(Q61L) (injected into the nucleus at 100 ug/mL). This image corresponds to Fig 2A and Video 2 in Cell Biol, 161:845-851, 2003. Video 2 is contained within the same image group as this figure.

Biological Sources

NCBI Organism Classification: Potorous tridactylus
Cell Type: epithelial cell
Cell Line: Pt K1 (NBL-3)
Cellular Component: cell; nucleus; lamellipodium; middle lamella

Biological Context

Molecular Function: active Rac

Attribution

Names: Torsten Wittmann; Gary M. Bokoch; Clare M. Waterman-Storer
Published: J Cell Biol, 161:845-851, 2003
Pubmed: 12796474

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL147
Archival Resource Key (ARK): ark:/b7295/w9cil147

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: phase contrast microscopy
Parameters Imaged: elastic scattering of photons
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	993px	——
Y	947px	——