Fluorescence images of control and nocodazole treated mitotic HeLa cells showing the effect of over expression of GFP-nuclear mitotic apparatus protein (NuMA) (blue) in relation to human structural maintenance of chromosomes (hSMC). Upper row, GFP control, second row, full-length NuMA, third and fourth rows MC and N NuMA mutants respectively. Cells were observed with an Olympus Introl, second row X70 microscope and recorded with a Magnaview CCD camera. See Fig 2E in Kong et al., 2009, Mol Biol Cell 20:1289-1301.