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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13876*  Cite 
Description

Tem1 (green) loading onto spindle pole bodies during metaphase is severely affected in bfa1Δ cells. Compare with control image CIL# 13877. DAPI (blue) and DIC (hidden) are also shown. CIL# 13871 and 13870 show corresponding anaphase images. Image is Fig S4B, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S4 include CIL# 13877, 13876, 13879, 13878.

Technical Details

Cells (MATa tem1::TEM1-yEGFP::SpHIS5 bfa1::HIS3) were fixed in 2.5% formaldehyde for 10 min, washed twice, and resuspended in 0.1 M potassium phosphate buffer, pH 6.4. Cells were then fixed for 10 min in 80% ethanol and resuspended in 1 mg/ml DAPI. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (Leica) and ImageJ software.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cell Line
W303
Cellular Component
nucleus
spindle pole body
cytoskeleton
cytoplasm
Attribution
Names
Mauricio Valerio-Santiago
Fernando Monje-Casas
Published
J Cell Biol. (2011) 192: 599-614.
Pubmed
21321099
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13876
Archival Resource Key (ARK)
ark:/b7295/w9cil13876
Grouping This image is part of a group.
Sample Preparation
Methods
formaldehyde fixed tissue
ethanol fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 187px 0.0642µm
Y 187px 0.0642µm