Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13654^* Cite

Description

Image is Fig. 4D in PMID: 20679433. HeLa cells were transfected to co-express p125A-mCherry and Sec31A-GFP. p125A colocalizes extensively with Sec31A; live cell time-lapse video microscopy showed that p125A (red) and Sec31A (green) positive structures are colocalized stably with each other over time, and undergo homotypic fusion. The vesicular structures of p125A-mCherry colocalize extensively with those of Sec31A-GFP over a period of at least 30 min. Cells were cultured on glass-bottom culture dishes, transfected with plasmid constructs of interest, and imaged using the Olympus Fluoview 1000 confocal microscope. 60X objective.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Line: HeLa
Cellular Component: trans-Golgi network

Biological Context

Biological Process: Golgi organization

Attribution

Names: Yan Shan Ong; Bor Luen Tang; Li ShenLoo; Wanjin Hong
Pubmed: PMID:20679433

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL13654
Archival Resource Key (ARK): ark:/b7295/w9cil13654

Grouping This image is part of a group.

Imaging

Image Type: confocal microscopy; bright-field microscopy
Image Mode: confocal microscopy; bright-field microscopy
Parameters Imaged: fluorescence emission; optical path length gradient
Source of Contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization Methods: Green fluorescent proteins from Aequorea; mCherryFP
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	——
Y	512px	——