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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13400*  Cite 
Description

A normal X. laevis blastomere expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). Single microtubules extend to the cortex, even in these large cells. X. laevis embryos were imaged on a laser scanning confocal microscope (1024; Bio-Rad Laboratories) mounted on an Axiovert microscope (Carl Zeiss, Inc.) using a 63× 1.4 NA Plan-Apochromat oil objective. Video corresponds to Supplementary Figure 1D and Video 2 from J Cell Biol. 2009 Dec 14;187(6):831-45.

Biological Sources
NCBI Organism Classification
Xenopus laevis laevis
Cell Line
Embryo
Cellular Component
microtubule
ensconsin
cell cortex
Biological Context
Biological Process
microtubule polymerization
Attribution
Names
George von Dassow
Koen J.C. Verbrugghe
Ann L. Miller
Jenny R. Sider
William M. Bement
Published
J Cell Biol. 2009 Dec 14;187(6):831-45.
Pubmed
20008563
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13400
Archival Resource Key (ARK)
ark:/b7295/w9cil13400
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 400px 0.3125µm
Y 300px 0.3125µm
Time 4 seconds 330