CIL:12637, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

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eukaryotic cell

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CIL:12637^* Cite

Description

Higher resolution image of the area that shows the clathrin-coated cage near the peniculus and clathrin coated preendosomal and partially coated preendosomal vesicles. Early endosomes like the preendosomal vesicles have smooth E-fracture faces with few IMPs. TEM taken on 7/19/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. Adapted with permission from the J. Cell Sci. The raw film was scanned with an Epson Perfection V750 Pro. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cytoplasm; early endosome; clathrin coat of endocytic vesicle

Biological Context

Biological Process: cytoplasm organization; endosome organization; clathrin coat assembly

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder
Published: J. Cell Sci. 101:449-461, 1992
Pubmed: 1629255
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12637
Archival Resource Key (ARK): ark:/b7295/w9cil12637

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; scanned image
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4258px	0.63nm
Y	3408px	0.63nm