Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12301^* Cite

Description

Another view of the cytopharynx but in a dividing Didinium. This cytopharynx is in the proter (anterior) daughter cell. The lamellae, each consisting of a set of perpendicularly arranged microtubules cover this food-vacuole forming region. Vesicles lie near the cytopharyngeal membrane and fuse with the membrane. TEM taken on 5/20/69 by R. Allen with Philips 300 operating at 60kV. Neg. 6,370X. Bar = 1µm.

Technical Details

The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A High resolution image is available in the library (CIL:38861) for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Didinium nasutum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Carolina Biological Supply Company, NC, U.S.A.
Cellular Component: cell cortex; cortical microtubule cytoskeleton

Biological Context

Biological Process: cortical cytoskeleton organization; cortical microtubule organization; plasma membrane organization
Molecular Function: structural constituent of cytoskeleton; structural molecule activity

Attribution

Names: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12301
Archival Resource Key (ARK): ark:/b7295/w9cil12301

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned to Photoshop.
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3557px	——
Y	2957px	——