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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12286^* Cite

Description

This confocal image of wild type cells plated on cell-derived matrices that correspond to an accompanying image of beta3-/- integrin cells. There are ncreased numbers of peripheral matrix-associated adhesions in the beta3-/- cells. The cells are co-stained for active beta1 integrin (9EG7-FITC, green), paxillin (Alexa-568, red) and fibronectin (Alexa-633, blue). Images were acquired using a Zeiss LSM 510 Meta microscope, 1024x1024 resolution, 13 stacks in the Z plane at 0.5um intervals. Images correspond to Fig 1C in J Cell Bio. 2010. 189:369-383

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: fibroblast
Cell Line: wild type corresponding to beta3 integrin -/-
Cellular Component: focal adhesion; extracellular matrix; cell derived 3D matrix

Biological Context

Biological Process

Attribution

Names: Daniel C. Worth; Kairbaan Hodivala-Dilke; Stephen D. Robinson; Samantha J. King; Penny E. Morton; Frank B. Gertler; Martin J. Humphries; Maddy Parsons
Published: J Cell Bio 2010. 189:369-383
Pubmed: 20404115

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12286
Archival Resource Key (ARK): ark:/b7295/w9cil12286

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Fluorescein (FITC); Alexa Fluor 568; Alexa Fluor 633; primary antibody plus labeled secondary antibody
Processing History: color combine
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1024px	0.127µm
Y	1024px	0.127µm
Z	——	0.5µm

Channel	Wavelength
1	488, 561, 633µm