Cell of the DT40 chicken B lymphocyte line at metaphase. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. In inner centromere protein INCENP-OFF cells, levels of phosphorylated histone H3 (H3-S10ph, red, and H3-S28ph, green) are low compared to INCENP-ON cells. DAPI stained chromosomes shown in blue. See Fig 2A panel 13 in Xu et al. 2009 J Cell Biol 187, 637-653.