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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12012*  Cite 
Description

Spontaneous symmetry breaking and motility initiation of keratocytes. Phase-contrast time-lapse video of a keratocyte spontaneously breaking symmetry and initiating motility. The cell has been reoriented such that the eventual direction of movement is to the right. 50x real time (frames were collected at 5-s intervals and are displayed at 10 frames/s). Cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic). Images were acquired every 5 s. Corresponds to Fig 2A (cell A0 and video 3 in JCB 178:1207-1221, 2007.

Biological Sources
NCBI Organism Classification
Hypsophrys nicaraguensis
Cell Type
keratocyte
Cellular Component
actin cytoskeleton
Biological Context
Biological Process
symmetry breaking
motility initiation
Attribution
Names
Patricia T. Yam
Cyrus A. Wilson
Lin Ji
Benedict Hebert
Erin L. Barnhar
Natalie A. Dye
Paul W. Wiseman
Gaudenz Danuser
Julie Theriot
Published
JCB 178:1207-1221, 2007
Pubmed
17893245
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12012
Archival Resource Key (ARK)
ark:/b7295/w9cil12012
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
contrast, brightness, image combine
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 327px 0.127µm
Y 310px 0.127µm
Time 5 seconds