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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11954*  Cite 
Description

The movie shows the effect of pressure on the spindle of the Chaetopteris egg. 100 Atm. of pressure induces depolymerization of spindle microtubules leading to the loss of birefringence in this polarizing microscopy study. As the spindle loses its birefringence and slowly shrinks, the chromosomes are pulled to the cell surface. When the pressure is returned to 1 Atm., the spindle microtubules reassemble and push the chromosomes away from the cell surface. The movie shows several rounds of pressure increases and decreases in which the birefingence of the spindle is clearly seen with polarizing optics. The last sequence shows the same cycle using phase contrast where the spindle is not so clearly seen.

Technical Details

The observations were made with polarizing and phase contrast optics using the hydrostatice pressure chamber developed by E.D. Salmon (Cold Spring Harbor Symp Quant Biol 3:1329-1342. See also: S Inoue et al. 1975.Functional organization of mitotic microtubules. Biophys J 15:725-744.

Biological Sources
NCBI Organism Classification
Chaetopterus sp. 1 Woods Hole MA
Cell Type
oocyte
Cellular Component
spindle
nuclear chromosome
Biological Context
Biological Process
meiosis
Attribution
Names
Shinya Inoue
Edward Salmon
Published
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11954
Archival Resource Key (ARK)
ark:/b7295/w9cil11954
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——