Alternate header for print version


Licensing
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11938*  Cite 
Description

The movie shows several sequences illustrating the generation of 3D data using the CSU-10 confocal microscope to image the auto-fluorescence of acid treated pollen grains of Dandelion. The first sequence is a 'walk through' in the z direction with the raw images at left, and after unsharp masking on the left. The images in the stack were displaced sidewise sequentially, unsharp masked, summed to generate the views for different angles, and sequences of angular views shown. In these latter sequences, it appears as though 3D images of pollen grains are rotating, providing insight on the complex structure.

Technical Details

See: S Inoue and T Inoue (2002) Direct-view high speed confocal scanner: the CSU-10. Meth Cell Biol 70:87-127

Biological Sources
NCBI Organism Classification
Taraxacum officinale
Cell Type
pollen grain
Attribution
Names
Shinya Inoue
Ted Inoue
Published
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11938
Archival Resource Key (ARK)
ark:/b7295/w9cil11938
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
Autofluorescence
Source of Contrast
differences in fluorescence emission
Visualization Methods
visualization of contiguous regions
Data Qualifiers
processed data
Sample Preparation
Methods
Acid treatment
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——