Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11831^* Cite

Description

Light microscopy of the same field of cells showing the phase contrast image of a monolayer of K2 Chinese Hamster Ovary (CHO)cells (upper panel), and in the lower panel, immunofluorescence staining using an antibody against a component of the nuclear lamina. Fluorescence is concentrated in the periphery of the nucleus. From Gerace L, Blum, Blobel G (1978) J. Cell Biol. 79, 546-56, reprinted with permission as Figures 159 (upper) and 160 (lower) from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the corresponding chapter is available on the ASCB's BioEDUCATE website.

Biological Sources

NCBI Organism Classification: Cricetulus griseus
Cell Line: CHO
Cellular Component: nucleus; nuclear lamina

Biological Context

Biological Process: nucleus organization

Attribution

Names: L. Gerace; A. Blum; G. Blobel
Published: ISBN 0721635849
Published: from: Don W. Fawcett M.D ‘The Cell’ 2nd edition, W.B. Saunders Chapter 4 (Nucleus)
Pubmed: 102651
Link: ASCB BioEDUCATE Program; Journal of Cell Biology

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL11831
Archival Resource Key (ARK): ark:/b7295/w9cil11831

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode: phase contrast microscopy; fluorescence microscopy
Parameters Imaged: fluorescence emission; distribution of epitope
Source of Contrast: differences in adsorption or binding of stain; boundaries between regions with different refractive index
Visualization Methods: Fluorescein (FITC)
Processing History: Image extracted from .pdf file of 'The Cell'
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: cell monolayer

Dimensions

Spatial Axis	Image Size	Pixel Size
X	865px	——
Y	1258px	——