Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11048^* Cite

Description

Nuclear pores are clearly seen in these transmission electron micrographs of cells after cryo-fixation, freeze-fracture and surface replication. Upper panel shows the outer surface of of the nuclear envelope from a guinea pig Sertoli cell. Lower panel shows the inner surface of the nuclear envelope of of a rat pancreatic acinar cell. Upper micrograph from Fawcett and Chemes (1979) Tissue Cell 11:147-162, reprinted as Figure 148 with permission; lower micrograph courtesy of Bernard Gilula is Figure 149 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the chapter is available on the ASCB's BioEDUCATE website.

Technical Details

See: Fawcett and Chemes (1979) Tissue Cell 11:147-162.

Biological Sources

NCBI Organism Classification: Cavia porcellus; Rattus rattus
Cell Type: Sertoli cell; pancreatic A cell
Cellular Component: nuclear envelope; nuclear pore

Biological Context

Biological Process: nucleus organization; nuclear transport; nuclear pore organization

Attribution

Names: Don W. Fawcett; Hector E. Chemes; Bernard Gilula
Published: ISBN 0721635849
Pubmed: 451991
Link: ASCB BioEDUCATE Program; Tissue and Cell
OTHER: from Don W. Fawcett M.D ‘The Cell’ 2nd edition, W.B. Saunders Chapter 4 (Nucleus)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL11048
Archival Resource Key (ARK): ark:/b7295/w9cil11048

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating
Processing History: Image extracted from .pdf file of 'The Cell'
Data Qualifiers: processed data

Sample Preparation

Methods: cryofixed tissue; freeze_fracture/freeze_etch
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	899px	——
Y	1296px	——