A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. Corresponds to raw data stack (without DIC): CIL# 10548 and deconvolved data stack: CIL# 10549. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
Spatial Axis | Image Size | Pixel Size |
---|---|---|
X | 1024px | 0.1067µm |
Y | 1024px | 0.1067µm |
Z | 19px | 0.3µm |