A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. Corresponds to raw data stack: CIL# 10538, maximum intensity projection stacks: CIL# 10540 and CIL# 10541 and raw data stack with DIC: CIL# 10543. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
Spatial Axis | Image Size | Pixel Size |
---|---|---|
X | 1024px | 0.1067µm |
Y | 1024px | 0.1067µm |
Z | 17px | 0.3µm |