Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10527^* Cite

Description

Live toxoplasma gondii cell line stably expressing EGFP fused to the beta II tubulin gene. Image was collected using ABI Deltavision on an Olympus PSF microscope with excitation at 450-490 nm and emission collected at 500-540 nm. Image was collected with a 60X water immersion objective, 1.2 N.A. and detected with a Cool Snap HQ/ICX285 camera. Associated raw data in image 10526.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii
Cell Line: toxoplasma gondii
Cellular Component: microtubule cytoskeleton

Biological Context

Biological Process: regulation of cell shape
Molecular Function: structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Names: John Murray; Ke Hu

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10527
Archival Resource Key (ARK): ark:/b7295/w9cil10527

Grouping This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: constrained iterative deconvolution
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.0711µm
Y	512px	0.0711µm
Z	12px	0.3µm