Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10521^* Cite

Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgMORN1 and mCherryFP-tagged Tgalpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Captured with CoolSnapHQ/ICX285. Deconvolved stack corresponds to raw data stack: CIL# 10520. Unpublished image that is similar to images published in PMID: 16518471 and 20140195. See publications for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: cortical microtubule; centriole; spindle pole; conoid; basal part of cell

Biological Context

Biological Process: regulation of cell shape; microtubule cytoskeleton organization
Molecular Function: structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Names: Ke Hu (Indiana University, Bloomington)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10521
Archival Resource Key (ARK): ark:/b7295/w9cil10521

Grouping This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP; mCherryFP
Processing History: 3D-deconvolution
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	128px	0.1326µm
Y	128px	0.1326µm
Z	11px	0.4µm