A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. 3D Deconvolved stack corresponds to raw data stacks: CIL# 10495 and 10494. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
Spatial Axis | Image Size | Pixel Size |
---|---|---|
X | 256px | 0.0663µm |
Y | 256px | 0.0663µm |
Z | 10px | 0.3µm |