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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10466*  Cite 
Description

Toxoplasma gondii expressing EGFP tagged Tgbeta2-tubulin and mCherry-TgDCCP (Double cortin containing protein), an unpublished microtubule binding protein. Images overlaid with DIC. For GFP, ex 450-490 em 500-540; for mCherry ex 555-592 em 600-665. Images acquired using API Delta Vision on a Olympus ix70 wide-field microscope. Objective: Olympus 60X water immersion, 1.2 NA. Captured with CoolSnapHQ/ICX285. 23 images in z-series; this is a deconvolved stack derived from raw data: CIL# 10465. Image is a control not treated with oryzalin. Part of an experiment with oryzalin-treatment. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources
NCBI Organism Classification
Toxoplasma gondii RH
Cell Line
Toxoplasma gondii RH stable cell line
Cellular Component
cortical microtubule
Biological Context
Biological Process
regulation of cell shape
Molecular Function
structural constituent of cytoskeleton
Attribution
Names
John Murray (University of Pennsylvania)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10466
Archival Resource Key (ARK)
ark:/b7295/w9cil10466
Grouping This image is part of a group.
Imaging
Image Type
recorded
charge coupled device (CCD)
Image Mode
fluorescence microscopy
differential interference contrast microscopy
Parameters Imaged
fluorescence emission
optical path length gradient
Source of Contrast
distribution of a specific protein
boundaries between regions with different refractive index
Visualization Methods
EGFP
mCherryFP
Processing History
3D-deconvolution
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 256px 0.1067µm
Y 256px 0.1067µm
Z 23px 0.3µm