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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10429*  Cite 
Description

Nucleated erythrocytes from chicken were spread on water, the dispersed chromatin picked up on carbon-formvar grids, fixed with paraformaldehye, critical point dried and shadowed with platinum. Images were obtained with the Wisconsin high voltage TEM at 1MEV using darkfield illumination. For this micrograph, the grid was tilted to 54 degrees. A similar micrograph tilted to 40 degrees providing a stereo pair with an oblique 3D view of the tangled and irregular chromatin fibers is grouped with this image. The combination of shadowing and darkfield illumination gives a quasi-negative stain effect revealing nucleosomes in some fibers.

Biological Sources
NCBI Organism Classification
Gallus gallus
Cell Type
erythrocyte
Cellular Component
nuclear chromatin
Attribution
Names
Hans Ris
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10429
Archival Resource Key (ARK)
ark:/b7295/w9cil10429
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
transmission electron microscopy (TEM)
darkfield illumination
Parameters Imaged
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
Processing History
unprocessed raw data
Sample Preparation
Methods
formaldehyde fixed tissue
critical_point dried specimen
Relation To Intact Cell
spread preparation
Dimensions
Spatial Axis Image Size Pixel Size
X 3647px 1nm
Y 2693px 1nm