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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10409*  Cite 
Description

DVD quality video of the Contractile Vacuole Complex of Paramecium multimicronucleatum. Measuring membrane tension of the in vitro CV. To measure the build up of tension by the CV membrane an in vitro CV surrounded by a thin layer of cytosol containing ATP was mounted on the tip of a holding pipette and a thin glass rod or sphere, mounted on the tip of a microcantilever, was rested on the CV's membrane. During rounding of the CV the sphere or glass rod was pushed upward which is then recorded as an output voltage of a position sensor. This can be converted into force which, in turn, can be converted into tension. The tension of the rounded CV membrane increased 35-fold over the tension of the relaxed membrane.

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
contractile vacuole
Biological Context
Biological Process
cellular water homeostasis
Attribution
Names
Tomomi Tani & Richard Allen
Pubmed
10607534
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10409
Archival Resource Key (ARK)
ark:/b7295/w9cil10409
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——