Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10102^* Cite

Description

Cultured retinal pigment epithelial cells immunofluorescently labeled for clathrin (green) and nucleus (blue). The cells were fixed in 2% PFA and 0.5% Triton X-100 for 2 minutes followed by post-fixation 4% PFA. Clathrin was detected with X22 primary antibody and secondary FITC antibody. The nucleus was detected with DAPI staining. Images were collected on an Olympus IX-71 epifluorescence microscope using a 100X 1.4 NA objective with 4.500ms exposure for clathrin and 50ms exposure for DAPI (67nm/pixel).

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell
Cell Line: ARPE-19
Cellular Component: clathrin coat; nucleus

Attribution

Names: Allen Liu; Sandra L. Schmid

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10102
Archival Resource Key (ARK): ark:/b7295/w9cil10102

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody; Fluorescein (FITC)
Processing History: unprocessed raw data

Sample Preparation

Methods: formaldehyde fixed tissue; permeabilized tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1344px	67nm
Y	1024px	67nm

Channel	Wavelength
1	350, 488nm