Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10064^* Cite

Description

Mammary epithelial MCF10A cells (immortalized normal mammary epithelial cells) transitioning from 2D to 3D collagen by grabbing and crawling on collagen-I fibers. scale bar = 50 microns. Time stamp Hrs:min:sec. Acquired in phase-contrast using a Plan Apo 20X 0.75 NA lens on a Nikon TE2000E inverted microscope. Celldance 2008, Honorable Mention Video

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell
Cell Line: MCF 10A
Cellular Component: cell; extracellular matrix

Biological Context

Biological Process: cell migration

Attribution

Names: Ghassan Mouneimne (Harvard Medical School)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10064
Archival Resource Key (ARK): ark:/b7295/w9cil10064

Imaging

Image Type: recorded image
Image Mode: phase contrast microscopy
Parameters Imaged: elastic scattering
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Methods: dispersed cells in vitro; cells in 3D matrix

Dimensions

Spatial Axis	Image Size	Pixel Size
X	540px	0.714µm
Y	410px	0.714µm

Time	150 seconds	12 hrs total