z-series image of mouse ES cells with a multi-copy alpha-globin BAC insertion. H3K9me3 (blue) marks the heterochromatic core which largely colocalizes with combined FISH signal from the lac operator ...

Image shows a z-series of a mouse ES cell carrying an alpha-globin BAC transgene array (clone 1c) hybridized with probes against the lac operator repeat (green) and the entire alpha-globin BAC sequenc...

Image shows a z-series immuno-FISH of a mouse ES cell carrying a multi-copy alpha-globin BAC insertion: HP1 gamma immunostaining (blue) is shown relative to BAC FISH signal (red) versus combined lac o...

Image shows an immuno-FISH z-series of a mouse ES cell carrying a multi-copy insertion of alpha-globin BAC showing partial co-localization of lac operator FISH signal (green) with H3K9me3 immunostaini...

z-series image of 3D immuno-FISH of multi-copy alpha-globin BAC insertions in a mouse ES cell. Immunostaining of HP1alpha (blue) is shown versus FISH signals against total BAC sequence (red) or combi...

Image shows a z-series of a mouse ES cell observed using 3D immuno-FISH. The ES cells carried a multi-copy insertion of an alpha-globin BAC. H3K9me3 immunostaining (red) is shown in comparison to vec...

Image shows a z-series of a mouse ES cell carrying a multi-copy alpha-globin BAC transgene array. Relative distribution of the BAC sequences, identified by DNA FISH (green) using the entire BAC as pr...

Z-series image of mouse ES cells carrying a beta-globin BAC transgene array (clone 8d) and transduced with a lentivirus containing an EGFP-lacI expression cassette. EGFP-LacI binding (green) to the 25...

Z-series images of mouse ES cells carrying DHFR BAC transgene array (clone 36) and differentiated into fibroblast-like cells. 3D FISH shows increase in number of lac operator foci (green) and change ...

Z-series image of mouse ES cells carrying an alpha-globin BAC transgene array (clone 7d) transduced with a lentivirus containing an expression cassette for EGFP-LacI. EGFP-LacI binding (green) to the...