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CCDB:83884*  Cite 
Project: P20196
Project name
Connexin43 APX
Description
Tomograms of specifically labeled connexin 43 (Cx43) gap junctions expressed in HEK293T. Cx43 was genetically tagged with GPF and APEX, an engineered plant peroxidase that reacts with hydrogen peroxide and diaminobenzidine to create a protein specific stain. Sections are cut perpendicular to the membrane plane (cross-section view).
Funding agency
NIH
Leader(s)
Gina Sosinsky
Collaborator(s)
Alice Ting
Jeff Martell
Mark Ellisman
Thomas Deerinck
Start date
02-16-2011
End date
unspecified
 
Experiment
Experiment ID
83871
Experiment date
02-23-2012
Title
Tomograms of Connexin 43
Purpose
Gap Junction Protein Cx43 tagged with GFP and APEX
Experimenter(s)
Andrea Thor
Tom Deerinck
Microscopy product
Microscopy product ID
83884
Instrument
JEOL_JEM4000EX-1
Microscopy type
TEM
Product type
DOUBLE TILT
Image basename
cnx43_40kx_tilt
Spatial Axis Image Size Pixel Size
X 3076px 0.376 nm/pixels
Y 3146px 0.376 nm/pixels
Y 251px
Subject
Species
Human
Scientific name
NA
Strain
NA
Age class
NA
Tissue section
Anatomical location
Kidney
Specimen description
Organ
kidney
Imaging parameters
Type
Electron microscopy product
Recording medium
4K CCD
Magification
40
Accelerating voltage
400 kev
Imaging product type
Type
Double tilt
X min range
-60 degrees
X max range
60 degrees
X tilt increment
2 degrees
Y min range
-60 degrees
Y max range
60 degrees
Y tilt increment
2 degrees