Image of a Caulobacter Crescentus specimen from dataset caulo2. Image is taken at 0 degrees from the tilt series.
Full resolution image description
All files necessary for reconstruction from tilt series to final tomogram using TxBR. Included are a .preali file, .fid, .rawtlt along with other relevant files and processing notes. Reconstruction using IMOD can still be done using these files.
Animation description
A .mpg file of the acquired and aligned tilt series of the caulobacter crescentus specimen
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3645
Instrument
JEOL 4000 #1
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
caulo2
Spatial Axis
Image Size
Pixel Size
X
2242px
1 nm/pixels
Y
3340px
1 nm/pixels
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus (NA1000)
Strain
NA1000
Treatment
None
Age class
N/A
Tissue section
Thickness
0.5 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
30000
Accelerating voltage
400 kV
Notes
Tilt series taken on JEOL 4000 #1
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees
Notes
Single tilt tomogram of a Caulobacter Crescentus Tomogram. HPF FS specimen #NA1000.
Citation Information
Lucy Shapiro, Harley McAdams, Patrick H. Viollier, Tom Deerinck, Mason Mackey, John Crum, Mark Ellisman (2003) CCDB:3645, Caulobacter crescentus (NA1000), Caulobacter crescentus. CIL. Dataset. https://doi.org/doi:10.7295/W9CCDB3645