Alternate header for print version


Reconstruction
Display image description
Image from the reconstructed volume of the Caulobacter Crescentus specimen. This particular image was taken from Z-slice #307
Full resolution image description
.tar file containing just the mrc file format of the tomographic reconstruction from the acquired tilt series.
Volume_dimension
970, 2868, 301
Animation description
A .mpg file of the slice-by-slice reconstructed volume of the Caulobacter Crescentus specimen.

Image 2D
Display image description
Image of a Caulobacter Crescentus specimen from dataset caulo2. Image is taken at 0 degrees from the tilt series.
Full resolution image description
All files necessary for reconstruction from tilt series to final tomogram using TxBR. Included are a .preali file, .fid, .rawtlt along with other relevant files and processing notes. Reconstruction using IMOD can still be done using these files.
Animation description
A .mpg file of the acquired and aligned tilt series of the caulobacter crescentus specimen

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3645*  Cite 
Project: P1220
Project name
Caulobacter
Description
Tomography of Caulobacter crescentus
Funding agency
Department of Energy
Leader(s)
Lucy Shapiro
Harley McAdams
Collaborator(s)
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Start date
07-01-2003
End date
unspecified
 
Experiment
Experiment ID
3374
Experiment date
05-12-2004
Title
Tomographic reconstruction of caulobacter
Purpose
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3645
Instrument
JEOL 4000 #1
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
caulo2
Spatial Axis Image Size Pixel Size
X 2242px 1 nm/pixels
Y 3340px 1 nm/pixels
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus (NA1000)
Strain
NA1000
Treatment
None
Age class
N/A
Tissue section
Thickness
0.5 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
30000
Accelerating voltage
400 kV
Notes
Tilt series taken on JEOL 4000 #1
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees
Notes
Single tilt tomogram of a Caulobacter Crescentus Tomogram. HPF FS specimen #NA1000.