SIngle slice from a through focus series through a medium spiny neuron from the dorsal neostriatum of a wild type mouse. Neuron was stained by intracellular injection of Lucifer Yellow, followed by photooxidation. The flocculent material surrounding the neuron likely represents photoconverted mitochondria or else debris generated by the photoconversion process.
Full resolution image description
Zip file containing through focus series in original BioRad PIC format and also converted to multiimage tiff format
Animation description
Animation stepping through a through-focus series of a medium spiny neuron from the dorsal neostriatum of a wild type mouse. Neuron was stained by intracellular injection of Lucifer Yellow, followed by photooxidation. The flocculent material surrounding the neuron likely represents photoconverted mitochondria or else debris generated by the photoconversion process.
Manual tracing of dendrites using Neurolucida. Because of the low magnification and photoconversion debris, the processes were sometimes difficult to follow.
Segmentation file description
Zip file containing Neurolucida trace file in ascii format (050803b_finaltrace.ASC), along with the output in VRML format. Summary files of measurements generated by Neuroexplorer for each of the parts traced are also included.