A contractile vacuole in systole. The CV has collapsed into vesicles and tubules. Three CV pores can be seen where the CV meets the pellicle. Smooth membrane cisterna surrounds some cytoplasm, possibly a developing stage of an autophagic vacuole. TEM taken on 6/12/69 by R. Allen with Philips 300 operating at 60kV. Neg. 9,250X. Bar = lµm. A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:39335) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.
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