Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9151^* Cite

Description

Transmission electron micrograph using immuno-gold labeling to show the location of PSD-95 in 3 glutamatergic synapses on an interneuron dendrite from the CA1 stratum radiatum of the hippocampus of an adult male rat (postnatal day 37). PSD-95 is labeled with a new rabbit monoclonal antibody (Cell Signaling Technology, Inc. - cat. #3450) and 10 nm immunogold (Ted Pella, GFAR). pre=presynaptic terminal, mit=mitochondrion, scale bar is 100 nm

Biological Sources

NCBI Organism Classification: Rattus
Cell Type: interneuron
Cellular Component: mitochondrion; pre synaptic terminal; dendrite; PSD-95

Biological Context

Biological Process: protein localization to synapse
Mouse Gross Anatomy: hippocampus

Attribution

Names: Ronald S. Petralia
OTHER: NIDCD/NIH

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9151
Archival Resource Key (ARK): ark:/b7295/w9cil9151

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: primary antibody plus labeled secondary antibody; gold
Processing History: unprocessed raw data

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2380px	——
Y	1964px	——