Licensing

Copyright
This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.
Comments: Powered by Pnyxe

Description

Mitosis is visualized here in a U2OS human osteosarcoma cell transduced with 'Cellular Lights' Histone 2B-RFP to mark the nucleus red and 'Cellular Lights' MAP4-GFP (MAP4 is a microtubule associated protein) and imaged the next day. In this video, microtubules appear green and histone proteins in the nucleus appear red. Cells were imaged on a Deltavision core microscope with a 40X objective images were collected every 5 minutes for 16 hours. Fluorescence was detected using the appropriate filters (FITC/TRITC). Scale bar = 20 microns.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Line
U-2 OS
Cellular Component
microtubule associated complex
nucleus

Biological Context

Biological Process
mitosis

Attribution

Names
Nick Dolman
Rob Batchelor
Penny Melquist
Mike O'Grady
George Hanson

Imaging

Image Type
recorded image
Imaging Mode
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
mRFP1
Processing History
deconvolution

Sample Preparation

Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 528px 0.16μm
Y 528px 0.16μm
Time 300 sec 578
*CIL – Cell Image Library accession number. Please use this to reference an image.