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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7753*  Cite 
Description

Mitosis is visualized here in a U2OS human osteosarcoma cell transduced with 'Cellular Lights' Histone 2B-RFP to mark the nucleus red and 'Cellular Lights' MAP4-GFP (MAP4 is a microtubule associated protein) and imaged the next day. In this video, microtubules appear green and histone proteins in the nucleus appear red. Cells were imaged on a Deltavision core microscope with a 40X objective images were collected every 5 minutes for 16 hours. Fluorescence was detected using the appropriate filters (FITC/TRITC). Scale bar = 20 microns.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
U-2 OS
Cellular Component
microtubule associated complex
nucleus
Biological Context
Biological Process
mitosis
Attribution
Names
Nick Dolman
Rob Batchelor
Penny Melquist
Mike O'Grady
George Hanson
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7753
Archival Resource Key (ARK)
ark:/b7295/w9cil7753
Imaging
Image Type
recorded image
Image Mode
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
mRFP1
Processing History
deconvolution
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 528px 0.16µm
Y 528px 0.16µm
Time 300 seconds 578


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