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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7747*  Cite 
Description

A high resolution view of cross-sectioned rows of basal bodies at the cortex of Nassula. Only a few basal bodies may have attached cilia as seen by the few cilia present in the upper right row. Basal bodies extend to the tips of the cytoplasmic mounds and each mound has a parasomal sac associated to one side. Unlike many other ciliates, the cartwheel occupies most of the basal body lumen. TEM taken on 4/1/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Nassula
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component
cell cortex
microtubule basal body
absence of cilia
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7747
Archival Resource Key (ARK)
ark:/b7295/w9cil7747
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 5833px 1nm
Y 4558px 1nm