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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7607*  Cite 
Description

High magnification view of rough endoplasmic reticulum (RER) from rat pancreas showing RER and ribosomes, both bound (RiB) and free (RiF). Ribosomes were originally called Palade particles, as Palade was the first to describe them. This micrograph, although not the original micrograph showing ribosomes, reflects Palade's continued fascination with the ribosome that he discovered in 1955. Identical unlabeled image available as CIL# 7606. Original report of Palade particles: Palade GE. A small particulate component of the cytoplasm. J Biophys Biochem Cytology [serial online, now J Cell Biol)]. 1955;1:59-68.

Technical Details

Small blocks of rat pancreas were fixed in 4% osmium tetroxide in 0.1 M cacodylate buffer pH 7.4 for 2 hours. The tissue was dehydrated and stained with uranyl acetate en block to bring out membrane structure and embedded in Epon. Sections were stained for 1 minute with acidic uranyl acetate and alkaline lead citrate for 5 minutes. 3.25 X 4 inch lantern slide scanned at 1200 dpi in TIFF format, labeled in Photoshop then reduced to 600 dpi TIFF file (3500 x 2969 pixels) prior to conversion to JPEG2000 format. Original image created on April 26, 1966.

Biological Sources
NCBI Organism Classification
Rattus rattus
Cell Type
pancreatic cell
Cellular Component
rough endoplasmic reticulum
cytosolic ribosome
Biological Context
Biological Process
biosynthetic process
Attribution
Names
George E. Palade (University of California, San Diego)
Link
Palade GE. 1955.
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7607
Archival Resource Key (ARK)
ark:/b7295/w9cil7607
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
lead salt
uranyl salt
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Dimensions
Spatial Axis Image Size Pixel Size
X 4800px 42.33µm
Y 4014px 42.33µm