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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7599*  Cite 
Description

This is a view of apical domains of two adjacent hepatocytes in the guinea pig liver, showing the junctional complexes that attach cells to one another. The bile canaliculus (BC) is defined by the cell membranes (CM) of two adjacent liver cells. At the level of the tight junctions (also known as zonula occludens, ZO) the intercellular spaces are sealed, thereby preventing leakage of bile from the canaliculus. Two adherens junctions (also known as zonula adherens, ZA) and two desmosomes (D) are seen distal (relative to the canaliculus) to each tight junction. Glycogen (gly) particles are apparent in the cytoplasm of the liver cells.This was part of the first description of tight junctions and junctional complexes between epithelial cells from many tissues. Identical unlabeled image available as CIL# 7598. Image published as Figure 13 in Farquhar and Palade, 1963.

Technical Details

Pieces of liver tissue were removed directly from anesthetized animals and immersed in a drop of fixative of 1% osmium tetroxide buffered at pH 7.6 with phosphate buffer. Tissue blocks were embedded in Epon. Sections were stained with uranyl acetate and lead hydroxide. Micrograph was taken at original magnification of 65,000 X with a Siemens Elmiskop I, operating at 60 or 80 kv, with a double condenser, and a 50 µ objective aperture. Digitization process: Lantern slide scanned at 1200 dpi in TIFF format, then reduced to 600 dpi TIFF file (3500 x 3067 pixels) prior to conversion to JPEG2000 format. Original resource provided as a 3.25 x 4 inch lantern slide by George E. Palade; original is in the Palade Collection, University of California, San Diego.

Biological Sources
NCBI Organism Classification
Cavia porcellus
Cell Type
hepatocyte
Cellular Component
tight junction
zonula adherens
desmosome
Biological Context
Biological Process
canalicular bile acid transport
Attribution
Names
George E. Palade
Published
J Cell Biol 17: 375-412. 1963
Pubmed
13944428
Link
Farquhar and Palade. 1963
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7599
Archival Resource Key (ARK)
ark:/b7295/w9cil7599
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
transmission electron microscopy (TEM)
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
lead salt
uranyl salt
Processing History
digitized lantern slide
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
osmium tetroxide fixed tissue
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 4800px 0.512nm
Y 4206px 0.512nm