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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7597*  Cite 
Description

High resolution image of cilia and cytopharyngeal vesicles containing a flocculent content of unknown composition which lie next to the cytopharynx and between the buccal cavity and what may be the nascent food vacuole. Such vesicles were not preserved in collidine-buffered fixative. TEM taken on 11/19/69 by R. Allen with Philips 300 operating at 60kV. Neg. 16,000X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Halteria grandinella
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Carolina Biological Supply Co., NC, U.S.A.
Cellular Component
cilium axoneme
vesicles of unknown function
food vacuole
Biological Context
Biological Process
ciliary or flagellar motility
digestion
unknown organelles
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7597
Archival Resource Key (ARK)
ark:/b7295/w9cil7597
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4986px 0.94nm
Y 6317px 0.94nm