Alternate header for print version


Licensing
This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7588*  Cite 
Description

This image shows the heterogeneity in the content of lysosomes (Lys) near the plasma membrane between two hepatocytes and near the bile canaliculus (not visible in this field). Some of the lysosomes contain residues resulting from partial digestion of lipids and some of these are more electron dense than others. Not only does the content of lysosomes vary depending on the cell type, but also physiological conditions, and even amongst lysosomes within an individual cell. Also in the field are mitochondria (Mt), rough endoplasmic reticulum (RER), and the cell membrane (CM). This image is part of a set of two; both images are identical, but on one of the twins, organelles are labeled by name. Small blocks of liver tissue were fixed in 4% osmium tetroxide in 0.1 M cacodylate buffer pH 7.4 for 2 hours. The tissue was dehydrated and embedded in Epon. Sections were stained for 1 minute with ethanolic uranyl acetate and then alkaline lead citrate for 5 minutes. Original resource was provided as a 3.25 x 4 inch lantern slide by George E. Palade; original is in the Palade Collection, University of California, San Diego. Digitization process: Lantern slide scanned at 1200 dpi in TIFF format, labeled in Photoshop then reduced to 600 dpi TIFF file (3500 x 2705 pixels) prior to conversion to JPEG2000 format.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
hepatocyte
Cellular Component
lysosome
Biological Context
Biological Process
lipid digestion
Molecular Function
lipid catabolic process
protein catabolic process
Attribution
Names
George E. Palade
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7588
Archival Resource Key (ARK)
ark:/b7295/w9cil7588
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Source of Contrast
lead salt
uranyl salt
Visualization Methods
detection of electrons
Data Qualifiers
processed data
Sample Preparation
Methods
osmium tetroxide fixed tissue
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 4800px ——
Y 3710px ——