Licensing
Description
A high resolution, longitudinal section through one toxicyst and one bundle of microtubules (nematodesma) of the oral area of Didinium involved in the killing and ingesting of large prey. Didinium specializes in consuming Paramecium, an organism nearly their own size in less than one minute at standard room temperature. This slightly oblique longitudinal section allows the opportunity to visualize the longitudinal substructure of the hexagonally packed microtubules that support the cytopharynx and assistin the process. TEM taken on 5/20/69 by R. Allen with Philips 300 operating at 60kV. Neg. 6,370X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Sources
- NCBI Organism Classification
- Didinium nasutum
- Cell Type
- eukaryotic cell
- Eukaryotic Protist
- Ciliated Protist
- Cell Line
- Carolina Biological Supply Company, NC, U.S.A.
- Cellular Component
- microtubule associated complex
- extrusive organelle
Biological Context
- Biological Process
- cortical microtubule organization
- killing of cells of other organism
Attribution
- Name
- Richard Allen
- Link
- http://www5.pbrc.hawaii.edu/allen/
Grouping
This image is part of a group.Imaging
- Image Type
- transmission electron microscopy (TEM)
- illumination by electrons
- Imaging Mode
- detection of electrons
- film
- Parameters Imaged
- electron density
- Source of Contrast
- elastic scattering
- Visualization Methods
- stain with broad specificity
- osmium tetroxide
- uranyl salt
- lead salt
- Processing History
- film
- Data Qualifiers
- raw, unprocessed data
- suitable for spatial measurements
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- osmium tetroxide fixed tissue
- tissue in epoxy resin embedment
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 3504px | 0.83nm |
| Y | 7801px | 0.83nm |