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High resolution image of the contractile vacuole and the spongiome decorated tubules showing V-type proton pumps extending from the cytosolic side of the tubules; also detail of the micronucleus. TEM taken on 6/10/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (

Biological Sources

NCBI Organism Classification
Opercularia [NCBITaxon:168247]
Opercularia coarctata
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component
integral to contractile vacuolar membrane
smooth endoplasmic reticulum

Biological Context

Biological Process
water transport
ionic transport
micronucleus organization
Molecular Function
v-type proton pumps
proton motive force dependent protein transmembrane transporter activity


Richard Allen


This image is part of a group.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements


Spatial Axis Image Size Pixel Size
X 5576px 1.0nm
Y 3757px 1.0nm
*CIL – Cell Image Library accession number. Please use this to reference an image.