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Description

HeLa cells at last stage of cytokinesis were fixed and stained with microtubules (green), PRC1 (red), and KIF4A (blue). PRC1 and KIF4A colocalized on midbody in the center of intracellular bridge and a dark zone which cannot be accessed by antibodies was shown. Cells were fixed with MeOH on ice for 3 min, and stained with primary antibodies against PRC1 (Protein regulator of cytokinesis 1), KIF4A (Chromosome-associated kinesin), and FITC-DM1a antibody against microtubules. Secondary antibodies were Alexa 594 for PRC1, and Alexa 647 for KIF4A. A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected with an OrCA ER CCD camera. Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, 568 Laser/ATOF 605/52, and 647 Laser ATOF 700/75.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
epithelial cell
Cell Line
HeLa
Cellular Component
microtubule
kinesin complex
midbody

Biological Context

Biological Process
cytokinesis
Molecular Function
microtubule motor activity

Attribution

Name
Hu, Chi-Kuo
Other
Tim Mitchison Lab

Grouping

This image is part of a group.

Sample Preparation

Methods
methanol fixed tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 914px 0.064μm
Y 875px 0.064μm
Channel Wavelength
1 488 nm
2 568 nm
3 647 nm
*CIL – Cell Image Library accession number. Please use this to reference an image.