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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7239*  Cite 
Description

HeLa cells were accumulated in monopolar mitosis using a 12 hr treatment of the kinesin-5 inhibitor S-trityl-l-cysteine and forced into cytokinesis by adding the potent Cdk1 inhibitor purvalanol A for 15 min. The microtubules (green) were not growing and shrinking. Aurora B (red), which functions in attaching microtubules to the centromere, localizes at the gap region between the cortex and the microtubule plus ends. Cells were fixed with MeOH on ice for 3 min, and stained with a primary antibody against Auruoa B and FITC-DM1a antibody against microtubules. Secondary antibody was Alexa 594 for Aurora B. A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected with an Orca ER CCD camera. Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, and 568 Laser/ATOF 605/52.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
Cell Line
HeLa
Cellular Component
microtubule
chromosome passenger complex
Biological Context
Biological Process
cytokinesis
Attribution
Names
Hu, Chi-Kuo
OTHER
Tim Mitchison Lab
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7239
Archival Resource Key (ARK)
ark:/b7295/w9cil7239
Grouping This image is part of a group.
Sample Preparation
Methods
methanol fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1114px 0.064µm
Y 901px 0.064µm
Channel Wavelength
1 488nm
2 568nm


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