Alternate header for print version


Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7224*  Cite 
Description

Distribution of Golgi Apparatus during cell division. Hela cells fixed with MeOH on ice for 3 min, and stained with a primary antibody against the Golgi and FITC conjugated DM1a antibody (green) against microtubules. Secondary antibody was Alexa 594 for the Golgi (red). A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected with an Orca ER CCD camera. Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, and 568 Laser/ATOF 605/52.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
Cell Line
HeLa
Cellular Component
Golgi apparatus
microtubule
Biological Context
Biological Process
cell division
Attribution
Names
Hu, Chi-Kuo
OTHER
Tim Mitchison Lab
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7224
Archival Resource Key (ARK)
ark:/b7295/w9cil7224
Grouping This image is part of a group.
Sample Preparation
Methods
methanol fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1344px 0.064µm
Y 1024px 0.064µm
Channel Wavelength
1 488nm
2 568nm