Licensing

Copyright
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Description

Mitotic spindle flattening. Compression elongates microtubule spindle. Widefield fluorescence images of Ptk2 cell expressing GFP tubulin flattened with a micromanipulator at 3:00-3:30 (min:s). Imaged with a 60X 1.4 NA objective on a Nikon TE300. Images captured with a Hamamatsu Orca ER. Filter set was HQ FITC Chroma, Ex 480/40 and Em 535/550. Bar is 5 microns. Corresponds to Video 1 in Curr Biol. 2009 Jul 14;19(13):1086-95

Biological Sources

NCBI Organism Classification
Macropus sp.
(kangaroo)
Cell Type
epithelial cell
Cell Line
PTK2
Cellular Component
spindle microtubule

Biological Context

Biological Process
mitotic spindle organization

Attribution

Names
Sophie Dumont
Timothy J Mitchison
Published
Curr Biol. 2009 Jul 14;19(13):1086-95
Pubmed
19540117

Imaging

Image Type
recorded image
Imaging Mode
fluorescence microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

Methods
unfixed tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 571px 0.2μm
Y 595px 0.2μm
Time 30 sec
*CIL – Cell Image Library accession number. Please use this to reference an image.