Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:6568^* Cite

Description

Mitotic spindle flattening. Compression elongates microtubule spindle. Widefield fluorescence images of Ptk2 cell expressing GFP tubulin flattened with a micromanipulator at 3:00-3:30 (min:s). Imaged with a 60X 1.4 NA objective on a Nikon TE300. Images captured with a Hamamatsu Orca ER. Filter set was HQ FITC Chroma, Ex 480/40 and Em 535/550. Bar is 5 microns. Corresponds to Video 1 in Curr Biol. 2009 Jul 14;19(13):1086-95

Biological Sources

NCBI Organism Classification: Macropus sp.
Cell Type: epithelial cell
Cell Line: PTK2
Cellular Component: spindle microtubule

Biological Context

Biological Process: mitotic spindle organization

Attribution

Names: Sophie Dumont; Timothy J Mitchison
Published: Curr Biol. 2009 Jul 14;19(13):1086-95
Pubmed: 19540117

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL6568
Archival Resource Key (ARK): ark:/b7295/w9cil6568

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; time lapse microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: unfixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	571px	0.2µm
Y	595px	0.2µm

Time	30 seconds